The newly formaldehyde biosensor is developed based on enzyme (nicotinamide adenine dinucleotide, NAD+, formaldehyde dehydrogenase, FDH) and nanomaterial (carbon nanotubes, nanoparticles, chitosan) for the detection of formaldehyde in fish samples.

The optimum scan rate was found at 0.5 V/s while the optimum pH 8 was used Tris-HCl buffer solution by using non-modified gold electrode via cyclic voltammetry technique. The response time was found within 5 second and concentration ranged between 1-10 ppm of formaldehyde. The formaldehyde biosensor is a promising tool and has a potential application for simple, fast, reusability, reproducibility, sensitivity, storage stability, validity, interferences and convenient method for the determination of formaldehyde in fish samples.