The identification of microorganisms have usually been based on conventional culturing techniques, which are time consuming, requiring 4-5 days for preliminary results and an additional 5 days for confirmation. DNA biosensor offers several advantages compared to existing techniques (e.g., rapid analysis, high throughput screening, increase detection limit, real time analysis and label-free detection).

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Electrochemical DNA biosensor is developed based on a gold electrode modified with an ionic liquid, nanoparticles and chitosan nanocomposite membrane. The modified electrode is immobilized of a single-stranded DNA probe. Methylene blue and acridine orange are used as an electrochemical indicator for monitoring the hybridization reaction of the target DNA sequence. This novel electrochemical biosensor is allowed the hybridization of synthetic target DNA, and also is permitted studies on the hybridization with crude DNA fragments and real samples (conidia and mycelia).