With high consumption of eggs, the waste by-products are either dumped at the dump site or burned for easy disposal. The gross majority of egg consumption takes place in the intensely populated region of Asia where this protein serves as a major food. The egg shell waste is available in huge quantity originating from food processing, beverage industry, hatching industries as well as from household. The egg shell goes as waste products and leads to pollution since it is microbial action on the environment and it is simply burned as organic waste that releasing carbon dioxide. By utilizing this organic waste that employed as platforms for the immobilization of enzymes and the lifespans of the immobilized enzymes are much extended.



Immobilization is the process of attaching enzyme to an insoluble material using physical or chemical interaction. Enzymes are recently highly demanded in many industrial applications and the demand for more stable, highly active and specific enzymes are growing rapidly. Immobilization enables the separation of the enzyme catalyst easily from the reaction mixture, and can lower the costs of enzymes dramatically. The present work is to characterize and fabricate the modified electrode based on ionic liquid/nanoparticles/eggshell membrane composite which concern about the characterization of egg shell membrane as well as to determine the selectivity, stability, sensitivity and detection limit of modified electrode by using enzymes which immobilized on egg shell membrane.